Journal: bioRxiv

Article Title: Kinetics of local C3 production orchestrates neutrophil recruitment in lung injury

doi: 10.64898/2026.02.17.706326

Figure Lengend Snippet: ( A ) Schematic of infection in mouse model. Created in BioRender. Kulkarni, H. (2026) https://BioRender.com/jevlom7 ( B-C ) Assay for detecting C3 activation on lipopolysaccharide (LPS) normalized to wildtype (WT) uninfected ( B ) bronchoalveolar lavage (BAL) and ( C ) serum of WT mice infected with Pseudomonas (Pa) and euthanized at 2, 4, 8, 12 and 24 h. Results for ( D ) full-length C3 ELISA and ( E ) C3a-neoepitope ELISA in BAL post- Pa infection. ( F ) Schematic of infection in ex vivo human lung model. Modified based on Zhang et al. ( G-I ) Changes in BAL ( G ) C3, ( H ) C3b/iC3b, and ( I ) IgM levels in ex vivo human lung pre-infection (T0, 0 h) and post-infection (T4, 4h). Distribution of data is represented as scatterplots showing individual data points, and bars represent means ± SD. ****P<0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05 using ordinary one-way ANOVA after adjusting for multiple group comparisons, and Mann-Whitney test for two-group comparison. Abbreviations: CFU: colony-forming units; FACS: fluorescence-associated cell sorting (flow cytometry); PEEP: positive end-expiratory pressure; RR: respiratory rate; VT: tidal volume.

Article Snippet: For use with BAL and serum, 96-well flat-bottom ELISA plates (#3855, Thermo Fisher Scientific) were coated with a C3b capture antibody (1:50 dilution; 100 μl per well in PBS; #HM1045, rat anti-mouse C3b/iC3b/C3d/C3dg, clone 11H9; Hycult Biotech) and incubated overnight at 4°C.

Techniques: Infection, Activation Assay, Enzyme-linked Immunosorbent Assay, Ex Vivo, Modification, MANN-WHITNEY, Comparison, Fluorescence, FACS, Flow Cytometry

Journal: Nucleic Acids Research

Article Title: RNA editing for the treatment of alpha-1 antitrypsin deficiency

doi: 10.1093/nar/gkaf1518

Figure Lengend Snippet: SERPINA1-994 decreases polymeric AAT accumulation in human iPSC-derived ZZ hepatocytes. ( A ) Images of cultured iPSCs after 14 days of treatment with the indicated AIMer. AAT polymer staining (green) and DAPI staining of nuclei (blue) are shown. The scale bars are 100 μm. ( B ) Quantification of polymeric AAT after 5, 10, or 14 days of treatment with increasing concentrations (0, 1.25, 2.5, 5.0, or 10 µM) of the indicated AIMer. Data are presented as mean ± SEM ( n = 6). White-adjusted two-way ANOVA with post-hoc comparisons to untreated allowing heteroscedasticity. ( C ) SERPINA1 RNA editing after 5, 10, or 14 days of treatment. Data are presented as mean ± SEM ( n = 4–6). White-adjusted two-way ANOVA with post-hoc comparisons to untreated allowing for heterscedasticity. ( D ) Quantification of nuclei per field after 5, 10, or 14 days. Data are presented as mean ± SEM ( n = 6). Two-way ANOVA with post-hoc comparisons to untreated. For all panels, P = 0.0 indicates that calculated P- values were below the limit of the program (2.2 × 10 −16 ). ns: nonsignificant.

Article Snippet: Fixed plates were immunostained with an antibody specific to the polymeric form of AAT (Hycult, Cat. No. HM2289) and quantified using a CellInsight CX7 high-content analysis platform (Thermo Fisher Scientific), and data were presented as mean fluorescence intensity per cell.

Techniques: Derivative Assay, Cell Culture, Polymer, Staining